CRISPR Screen Dataset

Wang LM (2023) - 2-PMID36652375

H. sapiens

Title: Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance.

Screen Rationale : Increased sensitivity to drug
Cell Type : Pancreatic Ductal Adenocarcinoma Cell Line [BTO:0003883]
Cell Line : Capan-1 [BTO:0002744, CELLOSAURUS:CVCL_0237]
Phenotype : Response To Chemicals
Condition : Thioparib (4-[[4-fluoro-3-[(5R)-5-methyl-3-(1,3-thiazol-2-yl)-6,8-dihydro-5H-[1,2,4]triazolo[4,3-a]pyrazine-7-carbonyl]phenyl]methyl]-2H-phthalazin-1-one) (50.0 nM) [PubChem]
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 256 | Full Dataset Size: 19,113
Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
Significance : Authors have indicated that results with Score.2 (p-Value) <= 0.05 AND Score.4 (sgRNA number) >= 3.0 AND Score.5 (Log2FC) <= -0.5 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

KO of the hit gene results in decreased proliferation in the presence of thioparib, suggesting the gene contributes to thioparib resistance

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