CRISPR Screen Dataset
Wang LM (2023) - 1-PMID36652375
Title: Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Pancreatic Ductal Adenocarcinoma Cell Line [BTO:0003883]
- Cell Line : Capan-1 [BTO:0002744, CELLOSAURUS:CVCL_0237]
- Phenotype : Response To Chemicals
- Condition : Thioparib (4-[[4-fluoro-3-[(5R)-5-methyl-3-(1,3-thiazol-2-yl)-6,8-dihydro-5H-[1,2,4]triazolo[4,3-a]pyrazine-7-carbonyl]phenyl]methyl]-2H-phthalazin-1-one) (50.0 nM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 256 | Full Dataset Size: 19,113
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) <= 0.01 AND Score.4 (sgRNA number) >= 3.0 AND Score.5 (Log2FC) >= 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased growth in the presence of thioparib, suggesting knock-out of the gene confers resistance
Score Distribution
< 1
> 4.2e-18