CRISPR Screen Dataset
Essletzbichler P (2023) - 2-PMID36725334
Title: A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
- Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
- Phenotype : Cell Proliferation
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DESeq2 | Number of Hits: 1,041 | Full Dataset Size: 18,195
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes results in decreased proliferation, suggesting the gene is essential for cell proliferation
Score Distribution
< -0.4
> -5.194