CRISPR Screen Dataset
Pavlou S (2023) - 1-PMID36894612
Title: CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neuronal cell death.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : IPSC Derived Cell Line [EFO:0005740]
- Cell Line : CAMi014-A [CELLOSAURUS:CVCL_A1NY]
- Phenotype : Response To Chemicals
- Condition : Tunicamycin (100.0 nM) [ChEBI]
- Library : CRISPRn Druggable Genome Library (Metzakopian, 2023) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 188 | Full Dataset Size: 4,391
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased growth in the presence of tunicamycin, suggesting the gene normally plays a role in tunicamycin sensitivity
Score Distribution
< 0.99998
> 0.0000616