CRISPR Screen Dataset
Branigan TB (2021) - 4-PMID33657372
Title: MMB-FOXM1-driven premature mitosis is required for CHK1 inhibitor sensitivity.
Screen Details:
- Screen Rationale : Decreased drug resistance
- Cell Type : Large Cell Lung Cancer Cell Line [BTO:0002205]
- Cell Line : NCI-H460 [BTO:0002207, CELLOSAURUS:CVCL_0459]
- Phenotype : Response To Chemicals
- Condition : Prexasertib (50.0 nM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 1,483 | Full Dataset Size: 19,114
- Experimental Setup : Drug Exposure | Duration: 19 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit genes causes decreased proliferation in the presence of CHK1 inhibitor prexasertib, suggesting the gene is required for CHK1i resistance in NSCLC cells
Score Distribution
< 6.998886939
> 1.313373701