CRISPR Screen Dataset
Wu X (2025) - 1-PMID40667754
Title: Identification of β4GALNT2 as an anti-hPIV3 factor through genome-wide CRISPR/Cas9 library screening.
Screen Details:
- Screen Rationale : Decreased resistance to virus
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Response To Virus
- Condition : HPIV-3 (Human parainfluenza 3 virus) (10.0 MOI) [NCBI Taxid 11216]
- Library : CRISPRn (Spaan, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 5 | Full Dataset Size: 20,446
- Experimental Setup : Virus Exposure | Duration: 20 Hours
- Significance : Authors have indicated that results with Score.1 (MAGeCK pos score) < 3e-05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased viral infection as measured by GFP, suggesting the gene normally plays an anti-viral role
Score Distribution
< 1
> 0.00000017986