CRISPR Screen Dataset
Xu P (2021) - 2-PMID33156908
Title: FBXO11-mediated proteolysis of BAHD1 relieves PRC2-dependent transcriptional repression in erythropoiesis.
Screen Details:
- Screen Rationale : Essential genes in immature erythroblasts
- Cell Type : Umbilical Cord Erythroid Progenitor
- Cell Line : HUDEP-2 [CELLOSAURUS:CVCL_VI06]
- Phenotype : Cell Proliferation
- Library : CRISPRn UPS targeted library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 46 | Full Dataset Size: 773
- Experimental Setup : Timecourse | Duration: 8 Days | MOI: ~ 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- From the text: Sequences with reduced representation after 8 days under expansion conditions represent proteins required for the survival and/or proliferation of immature erythroblasts
- Hits determined by Mageck but quantitative results for full screen only provided as log2fc and p-value
Score Distribution
< 1.206341407
> -4.018781378