CRISPR Screen Dataset
Soriaga LB (2025) - 9-PMID40372436
Title: Shared host genetic landscape of respiratory viral infection.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Colonic Cancer Cell Line [BTO:0000797]
- Cell Line : HCT 8 [CELLOSAURUS:CVCL_2478, BTO:0002255]
- Phenotype : Response To Virus
- Condition : Virus: HCoV-OC43 (Human coronavirus OC43) (0.01 MOI) [NCBI Taxid 31631]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 201 | Full Dataset Size: 20,196
- Experimental Setup : Virus Exposure | Duration: 96 Hours
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive score are enriched in a population exposed to the virus suggesting KO of the gene results in increased survival and the gene normally play a pro-viral role
- Genes with a significant negative score are depleted in a population exposed to the virus suggesting KO of the gene results in decreased survival and the gene normally play an anti-viral role
Score Distribution
< 0.99991
> 0.0000000000000020624