CRISPR Screen Dataset
Schmidt R (2022) - 4-PMID35113687
Title: CRISPR activation and interference screens decode stimulation responses in primary human T cells.
Screen Details:
- Screen Rationale : Regulation of stimulation-responsive cytokine production
- Cell Type : T Cell [CL:0000084]
- Cell Line : Primary T-cells
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Interleukin-2 (IL-2) (500.0 IU/ml) [Uniprot]
- Library : Human CRISPRi sgRNA library Dolcetto [AddGene, AddGene, AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 203 | Full Dataset Size: 18,939
- Experimental Setup : Cytokine exposure | Duration: 6 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 AND Score.5 (Log2FC) > 0.5 OR Score.4 (FDR) < 0.05 AND Score.5 (Log2FC) < -0.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a log2fc>0.5 and FDR<0.05 are enriched upon inhibition in a population sorted for IFN-gamma-producing CD8+ T cells, suggesting the gene is a negative regulator of IFN-gamma-production in primary cells
- Hit genes with a log2fc<-0.5 and FDR<0.05 are depleted upon inhibition in a population sorted for IFN-gamma-producing CD8+ T cells, suggesting the gene is positive regulator of IFN-gamma-production in primary cells
- The screen duration (6 days) is calculated as the time from infection with the sgRNA library to harvest of the cells for FACS. Cells were not exposed to IL-2 stimulation for the full duration of the screen.
Score Distribution
< 1
> 8.92e-25