CRISPR Screen Dataset
Findlay S (2023) - 1-PMID36920785
Title: The mitochondrial pyruvate carrier complex potentiates the efficacy of proteasome inhibitors in multiple myeloma.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Immortal Human Peripheral Neuron Cell Line [CLO:0000523]
- Cell Line : U-266 cell [CLO:0009457, CELLOSAURUS:CVCL_0566]
- Phenotype : Response To Chemicals
- Condition : Bortezomib (3.0 nM) [ChemSpider]
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 2,154 | Full Dataset Size: 15,652
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: = 0.2
- Significance : Authors have indicated that results with Score.1 (Beta Score) < -0.2 OR Score.1 (Beta Score) > 0.2 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative beta score show increased sensitivity to bortezomib upon KO
- Genes with a significant positive beta score show decreased sensitivity to bortezomib upon KO
Score Distribution
< 0.79139
> -0.90793