CRISPR Screen Dataset
Wright S (2023) - 3-PMID37036970
Title: Interrogating bromodomain inhibitor resistance in KMT2A-rearranged leukemia through combinatorial CRISPR screens.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : OCI-AML-2 [BTO:0004619, CELLOSAURUS:CVCL_1619]
- Phenotype : Response To Chemicals
- Condition : dBet1 (10.0 nM) [PubChem]
- Library : Human CRISPR Knockout Pooled Library (H3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1 | Full Dataset Size: 19,074
- Experimental Setup : Drug Exposure | Duration: 2 Weeks | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a significant positive MAGeCK score show increased resistance to PROTAC dBet1 upon knock-out
- Hit genes with a significant negative MAGeCK score show decreased resistance to PROTAC dBet1 upon knock-out
Score Distribution
< 0.99998
> 0.0000000635