CRISPR Screen Dataset
Zhao H (2021) - 2-PMID34879274
Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 TÂ cell expansion and anti-tumor immunity.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Cytotoxic T-lymphocyte (CD8+ T Cells) [BTO:0000289]
- Cell Line : OT-I cells
- Phenotype : Cell Proliferation
- Condition : Interleukin-2 (IL-2) [Uniprot]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,555 | Full Dataset Size: 19,674
- Experimental Setup : Cytokine exposure | Duration: 7 Days | MOI: ~ 0.5
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Hit genes represent sgRNAs that are depleted in OT-I cells, suggesting that KO of the gene results in decreased growth and the gene is essential for normal CD8 T cell expansion in vitro
Score Distribution
< -0.57808452
> -6.59068084