CRISPR Screen Dataset
Tsai PL (2022) - 1-PMID36417855
Title: Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins.
Screen Details:
- Screen Rationale : Regulators of inner nuclear membrane (INM) protein degradation
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 89 | Full Dataset Size: 19,112
- Experimental Setup : Timecourse | Duration: 13 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in decreased inner nuclear membrane (INM) protein degradation as measured by accumulation of LBR1600*S11, resulting in higher GFP fluorescence upon association with NLS-GFP1-10 in the nucleus
Score Distribution
< 1
> 4.91e-16