Screen Summary
Morgens DW (2017) - 4-PMID28474669
Title: Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens.
Screen Details:
- Screen Type : Positive Selection | Format: Pool
- Cell Line : K-562 cell | Type: Chronic Myeloid Leukemia Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Toxin Resistance
- Experimental Setup : Toxin Exposure | Duration: 14 Days | Condition Name: Ricin (0.25 ng/mL)
- Library : Bassik Human CRISPR Knockout Library | Type: CRISPRn | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 895 | Full Dataset Size: 20,440
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- authors used a cut-off of FDR<= 10% to determine significance
Score Distribution
< 526
> 0