CRISPR Screen Dataset
Morgens DW (2017) - 4-PMID28474669
Title: Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Response To Toxin
- Condition : Ricin (0.25 ng/mL)
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 895 | Full Dataset Size: 20,440
- Experimental Setup : Toxin Exposure | Duration: 14 Days
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- authors used a cut-off of FDR<= 10% to determine significance
Score Distribution
< 526
> 0