CRISPR Screen Dataset
Lau MT (2020) - 22-PMID32028983
Title: Systematic functional identification of cancer multi-drug resistance genes.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Response To Chemicals
- Condition : Vorinostat/SAHA (10.0 µM) [ChemSpider]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 3 | Full Dataset Size: 20,658
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR/Cas9 knockout screen was carried out to identify genes required for the cytotoxicity of anti-cancer drugs. Seven compounds (imatinib, olaparib, obatoclax, PAC-1, paclitaxel, RO-3066, and sorafenib) did not yield significant hits.
Score Distribution
< 0.999967
> 0.00165