CRISPR Screen Dataset
Lau MT (2020) - 1-PMID32028983
Title: Systematic functional identification of cancer multi-drug resistance genes.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Viability [APO:0000111]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 376 | Full Dataset Size: 20,674
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR/Cas9 screen was performed in Hap-1 cells to identify cell-essential genes affecting cell growth or viability in the absence of any drug.
Score Distribution
< 1
> 0.000495