CRISPR Screen Dataset
Chen Y (2024) - 1-PMID38885264
Title: MicroRNA-focused CRISPR/Cas9 screen identifies miR-142 as a key regulator of Epstein-Barr virus reactivation.
Screen Details:
- Screen Rationale : Regulation of EBV lytic reactivation
- Cell Type : Burkitt Lymphoma Cell Line [BTO:0000889]
- Cell Line : Mutu-1 [CELLOSAURUS:CVCL_7202]
- Phenotype : Response To Virus
- Condition : anti-IgM (10 µg/mL)
- Library : LX-miR gRNA library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 39 | Full Dataset Size: 1,594
- Experimental Setup : Other | Duration: 9 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive DrugZ score are enriched in a gp350 (late viral envelope protein)-positive population, suggesting that KO of the miRNA results in increased lytic activation of EBV and the gene normally restricts EBV reactivation
- Genes with a significant negative DrugZ score are depleted in a gp350 (late viral envelope protein)-positive population, suggesting that KO of the miRNA results in decreased lytic activation of EBV and the gene normally contributes to EBV reactivation
Score Distribution
< 5.97
> -3.22