CRISPR Screen Dataset
Yang H (2022) - 1-PMID35804923
Title: Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic Cancer.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Pancreatic Cancer Cell Line [BTO:0000794]
- Cell Line : TB32047
- Phenotype : Response To Chemicals
- Condition : Gemcitabine (50.0 nM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 3 | Full Dataset Size: 22,507
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) > 0.0 AND Score.2 (p-Value) < 0.05 AND Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased growth in the presence of gemcitabine
Score Distribution
< 1
> 0.000000000003093