CRISPR Screen Dataset
Stover EH (2019) - 8-PMID31462500
Title: Pooled Genomic Screens Identify Anti-apoptotic Genes as Targetable Mediators of Chemotherapy Resistance in Ovarian Cancer.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Ovary Adenocarcinoma Cell Line [BTO:0001882]
- Cell Line : OVSAHO [CELLOSAURUS:CVCL_3114]
- Phenotype : Response To Chemicals
- Condition : Cisplatin and paclitaxel (taxol) [ChemSpider, ChemSpider]
- Library : Avana-4 | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,101 | Full Dataset Size: 18,901
- Experimental Setup : Drug Exposure | Duration: 14 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a significant positive LFC show increased resistance to cisplatin and paclitaxel (0.5 uM + 10 nM) upon KO
- Hit genes with a significant negative LFC show increased sensitivity to cisplatin and paclitaxel (0.5 uM + 10 nM) upon KO
Score Distribution
< 1.886446263
> -2.12585221