CRISPR Screen Dataset
Zelceski A (2023) - 4-PMID37163373
Title: MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : MCF-10A [BTO:0001939, CELLOSAURUS:CVCL_0598]
- Phenotype : Response To Chemicals
- Condition : talazoparib (25.0 nM) [PubChem]
- Library : Weissman Human CRISPRi Titration Libraries [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Z-score | Number of Hits: 858 | Full Dataset Size: 17,810
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Inhibition of the hit gene results in decreased proliferation in the presence of talazoparib in MCF10A TP53-/- cells
Score Distribution
< 12.09739904
> -28.45904762