CRISPR Screen Dataset
Huang K (2019) - 1-PMID31508283
Title: Genome-Wide CRISPR-Cas9 Screening Identifies NF-κB/E2F6 Responsible for EGFRvIII-Associated Temozolomide Resistance in Glioblastoma.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Glioblastoma Cell Line [BTO:0001530]
- Cell Line : U-87MG ATCC [BTO:0002036, CELLOSAURUS:CVCL_0022]
- Phenotype : Response To Chemicals
- Condition : Temozolomide (375.0 µM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,289 | Full Dataset Size: 20,914
- Experimental Setup : Drug Exposure | Duration: 7 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes with a significant negative logfc results in increased sensitivity to temozolomide in U87-EGFRvIII cells
- KO of hit genes with a significant positive logfc results in decreased sensitivity to temozolomide in U87-EGFRvIII cells
Score Distribution
< 3.407818542
> -7.194949419