CRISPR Screen Dataset
Lin K (2024) - 10-PMID38291084
Title: A scalable platform for efficient CRISPR-Cas9 chemical-genetic screens of DNA damage-inducing compounds.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Camptothecin (20.0 IC) [ChemSpider]
- Library : Targeted subset of TKOv3 (Myers, 2024) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Differential LFC | Number of Hits: 133 | Full Dataset Size: 1,010
- Experimental Setup : Drug Exposure | Duration: 12 Days | MOI: = 0.2
- Significance : Authors have indicated that results with Score.1 (CGI) < -0.7 AND Score.3 (FDR) < 0.1 OR Score.1 (CGI) > 0.7 AND Score.3 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- hTERT-immortalized RPE-1 TP53 knockout cell were screened in the presence of camptothecin using a targeted library of genes from four categories: well-characterized DNA damage response genes, genes that captured the greatest variance across published CRISPR screens, genes that captured subtle fitness defects in CRISPR screen data, and genes that have a high degree of genetic interactions selected from a pool of guides from the genome-wide Toronto KnockOut version 3.0 (TKOv3) library
- Genes with a significantly negative CGI score show increased sensitivity to camptothecin upon KO
- Genes with a significantly positive CGI score show increased resistance to camptothecin upon KO
Score Distribution
< 3.442704075
> -3.076029503