CRISPR Screen Dataset
Yan K (2024) - 1-CUSTOM00000027
Title: TMEM106B-mediated SARS-CoV-2 infection allows for robust ACE2-independent infection in vitro but not in vivo
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK-293 [BTO:0000007, CELLOSAURUS:CVCL_0045]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 mouse adapted 1 (SARS-CoV-2MA1) (Spike E484D) (Yan, 2022) (0.1 MOI)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 128 | Full Dataset Size: 19,113
- Experimental Setup : Virus Exposure | Duration: 3 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.2 (FDR) < 0.05 OR Score.3 (Log2FC) < 0.0 AND Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out to identify host factors that facilitate ACE2-independent infection for SARS-CoV-2(MA1) which contains an E484D amino acid substitution in the spike protein.
Score Distribution
< 10.066
> -9.0192