CRISPR Screen Dataset
Morris SNS (2022) - 1-PMID36409314
Title: A genome-wide CRISPR screen implicates plasma membrane asymmetry in exogenous C6-ceramide toxicity.
Screen Details:
- Screen Rationale : Increased/decreased ceramide resistance
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : C6 ceramide (30.0 µM) [PubChem]
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 122 | Full Dataset Size: 20,501
- Experimental Setup : Other | Duration: 12 Days
- Significance : Authors have indicated that results with Score.1 (CasTLE Score) > 25.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a positive Castle Effect show increased resistance to C6-Cer toxicity
- Hit genes with a negative Castle Effect show increased sensitivity to C6-Cer toxicity
Score Distribution
< 301
> 0