Screen Summary
Meyers RM (2017) - 52-PMID29083409
Title: Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells.
Screen Details:
- Screen Type : Negative Selection | Format: Pool
- Cell Line : H4 neuroglioma cell | Type: Glioma Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Viability
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: < 1
- Library : Avana | Type: CRISPRn | Methodology: Knockout
- Analysis Method : CERES | Number of Hits: 1,752 | Full Dataset Size: 17,670
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- To identify gene hits at an FDR < 0.05, ORCS estimated FDRs for the CERES Avana dataset using customized gold-standard positive and negative sets of genes. The custom positive set included genes essential in at least 90% of cell lines in both the CERES Avana dataset (PMID:29083409) and the 10 lines included in Bertomeu et al. (PMID:29038160). The custom negative set included genes essential in zero cell lines from one of the two datasets and in <=10% of the other dataset.
Score Distribution
< 2.88810627978
> -1.79330378441