CRISPR Screen Dataset
Wang C (2021) - 8-PMID34320214
Title: Genetic vulnerabilities upon inhibition of DNA damage response.
Screen Details:
- Screen Rationale : Decreased drug resistance
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293-A [BTO:0002524, CELLOSAURUS:CVCL_6910]
- Phenotype : Response To Chemicals
- Condition : Olaparib|AZD0156 [PubChem, PubChem]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 30 | Full Dataset Size: 17,929
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -3.0 AND Score.2 (p-Value) < 0.015 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are synthetic lethal when combined with PARP inhibition (Olaparib, 1 uM) and ATM inhibition (AZD0156, 0.5 uM)
Score Distribution
< 6.06
> -5.8