CRISPR Screen Dataset
Liu Y (2022) - 2-PMID36396656
Title: mTOR inhibition attenuates chemosensitivity through the induction of chemotherapy resistant persisters.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pancreatic Ductal Adenocarcinoma Cell Line [BTO:0003883]
- Cell Line : 4292 KRASG12D/p53R172H
- Phenotype : Response To Chemicals
- Condition : selinexor (0.33 µM) [ChEBI]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 3,483 | Full Dataset Size: 19,674
- Experimental Setup : Drug Exposure | Duration: 12 Days
- Significance : Authors have indicated that results with Score.1 (Beta Score) >= 0.1188 OR Score.1 (Beta Score) < -0.1893 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with significant positive beta score show increased resistance to selinexor upon KO
- Genes with significant negative beta score show increased sensitivity to selinexor upon KO
Score Distribution
< 3.0709
> -2.8743