CRISPR Screen Dataset
Tuano NK (2023) - 49-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Response To Chemicals
- Condition : Olaparib (5.0 µM) [PubChem]
- Library : CRISPRi targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 28 | Full Dataset Size: 1,956
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative log2fc cause increased sensitivity to olaparib upon inhibition
- Genes with a significant positive log2fc cause decreased sensitivity to olaparib upon inhibition
- screen performed in HMEC cells with a mesenchymal signature (mesHMLE)
- authors used a cut-off of -1>log2fc>1 and log10FDR>1
Score Distribution
< 2.5381
> -2.7135