CRISPR Screen Dataset
Tuano NK (2023) - 47-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Response To Chemicals
- Condition : Olaparib (5.0 µM) [PubChem]
- Library : CRISPRn targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 21 | Full Dataset Size: 1,949
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative log2fc cause increased sensitivity to olaparib upon KO
- Genes with a significant positive log2fc cause decreased sensitivity to olaparib upon KO
- screen performed in HMEC cells with a luminal progenitor signature (K5+/K19-)
- authors used a cut-off of -1>log2fc>1 and log10FDR>1
Score Distribution
< 1.5664
> -3.7547