CRISPR Screen Dataset
Tuano NK (2023) - 41-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Tumor promoting genes
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Tumorigenicity
- Condition : Mutation: MAP2K1 (ETG5604) constitutively active form of MEK1 (MEKDD)
- Library : CRISPRa targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRa | Format: in vivo | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 2 | Full Dataset Size: 1,959
- Experimental Setup : Implantation to Mouse Model | Duration: 42 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive log2fc cause increased tumor formation upon activation, suggesting the gene plays an oncogenic role
- screen performed in HMEC cells with an epithelial like signature (B80-T5) implanted into a mouse model
- authors used a cut-off of log2fc>1 and log10FDR>1
Score Distribution
< 6.1914
> -10.54