CRISPR Screen Dataset
Kwanten B (2023) - 10-PMID36731340
Title: E3 ubiquitin ligase ASB8 promotes selinexor-induced proteasomal degradation of XPO1.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Diffuse Large B-cell Lymphoma Cell [BTO:0002521]
- Cell Line : RL [CELLOSAURUS:CVCL_1660, BTO:0003230]
- Phenotype : Response To Chemicals
- Condition : selinexor (40.0 nM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CRISPRBetaBinomial | Number of Hits: 161 | Full Dataset Size: 19,115
- Experimental Setup : Drug Exposure | Duration: 25 Days | MOI: ~ 0.25
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a positive lfc show increased resistance to selinexor
- Hit genes with a negative lfc show increased sensitivity to selinexor
Score Distribution
< 3.574126486
> -2.749042374