CRISPR Screen Dataset
Gilliland HN (2023) - 1-PMID36794958
Title: A Genome-Wide Screen in Macrophages Defines Host Genes Regulating the Uptake of Mycobacterium abscessus.
Screen Details:
- Screen Rationale : Increased resistance to bacterial uptake
- Cell Type : Macrophage [BTO:0002278]
- Cell Line : iBMDM (immortalized bone marrow derived macrophages) [CELLOSAURUS:CVCL_C0MW]
- Phenotype : Response To Bacteria
- Condition : Mycobacterium abscessus (5.0 MOI) [NCBI Taxid 36809]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Robust-rank aggregation (RRA) | Number of Hits: 100 | Full Dataset Size: 11,702
- Experimental Setup : Bacteria Exposure | Duration: 4 Hours
- Significance : Authors have indicated that results with Score.1 (Rank) <= 100.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are enriched in a population sorted for low Mycobacterium abscessus uptake, suggesting KO of the hit gene prevents uptake
- Authors used a combination of gene level p-values and sgRNA level log2fc values to determine hits. The top 100 ranked genes from their results met these thresholds.
Score Distribution
< 11702
> 1