CRISPR Screen Dataset
Wei W (2023) - 1-PMID36820830
Title: Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : MOLM-13 [BTO:0004175, CELLOSAURUS:CVCL_2119]
- Phenotype : Response To Chemicals
- Condition : SHP099 (5.0 µM) [ChemSpider]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 18,051
- Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.4 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive MaGeCK score show increased resistance to SHP2 inhibition with SHP099
- Genes with a significant negative MaGeCK score show increased sensitivity to SHP2 inhibition with SHP099
Score Distribution
< 1
> 0.0000000000895