CRISPR Screen Dataset
Heo JM (2019) - 2-PMID31723608
Title: Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.
Screen Details:
- Screen Rationale : Negative regulators of mitophagic flux
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein Transport [APO:0000129]
- Condition : antimycin A/oligomycin A []
- Library : Resident mitochondrial targeted library (Elledge and Harper, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 111 | Full Dataset Size: 1,094
- Experimental Setup : Drug Exposure | Duration: 175 Hours | MOI: = 0.2
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 AND Score.4 (Log2FC) > 0.28 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are enriched in a population of cells with high levels of the mitophagic flux reporter mt-mkeima, suggesting that KO of the gene results in increased mitophagic flux
Score Distribution
< 0.99999
> 0.0000000000000215