CRISPR Screen Dataset
Mengwasser KE (2019) - 2-PMID30686591
Title: Genetic Screens Reveal FEN1 and APEX2 as BRCA2 Synthetic Lethal Targets.
Screen Details:
- Screen Rationale : Cell-essential and growth restrictive genes in a BRCA2 mutant
- Cell Type : Ovarian Cancer Cell Line [BTO:0000811, EFO:0002394]
- Cell Line : PEO1 [EFO:0005445, CELLOSAURUS:CVCL_2686]
- Phenotype : Cell Proliferation
- Condition : Mutation: BRCA2 (ETG675) KO
- Library : DDR targeted library (Elledge, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE/EdgeR | Number of Hits: 125 | Full Dataset Size: 357
- Experimental Setup : Timecourse | Duration: 12 Doublings
- Significance : Authors have indicated that results with Score.4 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a negative score are synthetic lethal with BRCA2. Hit genes with a positive score suppress the growth defects seen in a BRCA2 mutant.
Score Distribution
< 1.6989
> -1.1794