CRISPR Screen Dataset
Zhang Z (2022) - 1-PMID35688159
Title: RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells.
Screen Details:
- Screen Rationale : Tumor cell survival in the presence of CD8+ T cells
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : D10 [CELLOSAURUS:CVCL_H945]
- Phenotype : Cell Proliferation
- Condition : CD8+ T cells (0.125 Ratio of effector cells/target cells)
- Library : GECKO | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 683 | Full Dataset Size: 20,530
- Experimental Setup : T cell exposure | Duration: 13 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (MAGeCK neg score) < 0.001 OR Score.2 (FDR) < 0.001 OR Score.3 (MAGeCK pos score) < 0.001 OR Score.4 (FDR) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative score are depleted in tumor cells exposed to CD8+ T cells, suggesting KO of the hit gene confers sensitivity to CD8+ T cells in tumor cells
- Genes with a significant positive score are enriched in tumor cells exposed to CD8+ T cells, suggesting KO of the hit gene confers resistance to CD8+ T cells in tumor cells
Score Distribution
< 1
> 0.000000532