CRISPR Screen Dataset

Sellar RS (2022) - 1-PMID35763353

H. sapiens

Title: Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells.

Screen Rationale : Increased/decreased drug resistance
Cell Type : Acute Lymphoblastic Leukemia Cell Line [BTO:0002144]
Cell Line : MM1.S [EFO:0005724, CELLOSAURUS:CVCL_8792]
Phenotype : Response To Chemicals
Condition : CC-885 (0.25 nM) [ChemSpider, PubChem]
Library : Avana-4 | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : EdgeR | Number of Hits: 1,876 | Full Dataset Size: 17,987
Experimental Setup : Drug Exposure | Duration: 16 Days
Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.3 (p-Value) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Significant genes with a positive Nscore are enriched in CC-885 treated samples suggesting KO of the gene confers resistance
Significant genes with a negative Nscore are depleted in CC-885 treated samples suggesting KO of the hit gene confers sensitivity

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