CRISPR Screen Dataset
Feng R (2022) - 1-PMID36224385
Title: Activation of γ-globin expression by hypoxia-inducible factor 1α.
Screen Details:
- Screen Rationale : Regulation of globin expression (HbF/HbA)
- Cell Type : Umbilical Cord Erythroid Progenitor
- Cell Line : HUDEP-2 [CELLOSAURUS:CVCL_VI06]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Differentiation Medium
- Library : CRISPRn UPS targeted library (Weiss, 2022) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 28 | Full Dataset Size: 774
- Experimental Setup : Other | Duration: 5 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for UPS genes that were enriched in an HbF expressing set of cells, suggesting that KO of the gene induces fetal hemoglobin (HbF) expression instead of adult hemoglobin (HbA) expression
Score Distribution
< 5.0068
> -6.2658