CRISPR Screen Dataset
Tsujino T (2023) - 4-PMID36650183
Title: CRISPR screens reveal genetic determinants of PARP inhibitor sensitivity and resistance in prostate cancer.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Prostate Cancer Cell Line [BTO:0001033]
- Cell Line : DU145 [BTO:0001332, CELLOSAURUS:CVCL_0105]
- Phenotype : Response To Chemicals
- Condition : Olaparib (10.0 µM) [PubChem]
- Library : Liu Human CRISPR Knockout Library (CRISPRn H1 H2, H1/H2) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeckFlute | Number of Hits: 281 | Full Dataset Size: 18,436
- Experimental Setup : Drug Exposure | Duration: 28 Days | MOI: ~ 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit genes results in increased resistance to Olaparib
Score Distribution
< 1.355755896
> 0.350381564