CRISPR Screen Dataset
Cheruiyot A (2021) - 1-PMID34215620
Title: Nonsense-Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells Harboring or Mutations.
Screen Details:
- Screen Rationale : Regulation of Nonsense Mediated Decay (NMD)
- Cell Type : Osteosarcoma Cell Line [BTO:0000407]
- Cell Line : U2OS [BTO:0001938, CELLOSAURUS:CVCL_0042]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 910 | Full Dataset Size: 18,902
- Experimental Setup : Timecourse | Duration: 6 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene is enriched in a pool of NMD inhibited cells as measured by a fluorescent reporter, suggesting the gene normally plays a role in promoting NMD
Score Distribution
< 0.99984
> 0.0000000411