CRISPR Screen Dataset
van der Weegen Y (2021) - 2-PMID34108663
Title: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation.
Screen Details:
- Screen Rationale : Cell essential genes in an ELOF1 KO background
- Cell Type : Embryonic Cell Line [EFO:0003040]
- Cell Line : HUES 62 [EFO:0007087, CELLOSAURUS:CVCL_B197]
- Phenotype : Cell Proliferation
- Condition : Mutation: ELOF1 (ETG84337) KO
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: iCAS9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 40 | Full Dataset Size: 18,056
- Experimental Setup : Timecourse | Duration: 12 Doublings | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes show synthetic lethality with ELOF1 KO
Score Distribution
< 8.98
> -10.74