CRISPR Screen Dataset
Baxter JS (2023) - 1-PMID37866880
Title: Cancer-associated FBXW7 loss is synthetic lethal with pharmacological targeting of CDC7.
Screen Details:
- Screen Rationale : Cell essential genes in an FBXW7 mutant background
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : MCF-10A [BTO:0001939, CELLOSAURUS:CVCL_0598]
- Phenotype : Cell Proliferation
- Condition : Mutation: FBXW7 (ETG55294) del
- Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score based on MAGECK score | Number of Hits: 462 | Full Dataset Size: 17,998
- Experimental Setup : Timecourse | Duration: 2 Weeks | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes that were synthetic lethal with an FBXW7 mutant (FBXW7-17) upon KO
- FBXW7-17 contained a 2093 bp deletion within FBXW7 (between genomic coordinates chr4:152328220 and 152330312)
Score Distribution
< 8.643160152
> -14.71759797