CRISPR Screen Dataset
Park JM (2023) - 1-PMID37047518
Title: Genome-Wide CRISPR Screens Reveal ZATT as a Synthetic Lethal Target of TOP2-Poison Etoposide That Can Act in a TDP2-Independent Pathway.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293-A [BTO:0002524, CELLOSAURUS:CVCL_6910]
- Phenotype : Response To Chemicals
- Condition : Etoposide (100.0 nM) [ChEBI]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 1,243 | Full Dataset Size: 17,928
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -7.0 OR Score.1 (Z-score) > 7.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with negative Z-score confer ETO sensitivity upon KO in wildtype cells
- Genes with a positive Z-score confer ETO resistance upon KO in wildtype cells
Score Distribution
< 13.35
> -64.38