CRISPR Screen Dataset
Normandin K (2023) - 2-PMID37639469
Title: Genetic enhancers of partial PLK1 inhibition reveal hypersensitivity to kinetochore perturbations.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pre-B Acute Lymphoblastic Leukemia Cell Line [BTO:0000603]
- Cell Line : NALM-6 [BTO:0003288, CELLOSAURUS:CVCL_0092]
- Phenotype : Response To Chemicals
- Condition : BI6727/Volasertib (12.5 nM) [PubChem]
- Library : Extended Knockout Library (EKO) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RANKS | Number of Hits: 23 | Full Dataset Size: 19,012
- Experimental Setup : Drug Exposure | Duration: 8 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a negative score are significantly depleted in the presence of PLK1 inhibitor BI6727/Volasertib upon KO suggesting loss of the gene sensitizes cells to PLK1 inhibition
- Hit genes with a positive score are significantly enriched in the presence of PLK1 inhibitor BI6727/Volasertib upon KO suggesting loss of the gene causes resistance to PLK1 inhibition
Score Distribution
< 4.04
> -4.59