CRISPR Screen Dataset
Zhu X (2020) - 1-PMID32668236
Title: Ribosome Recycling by ABCE1 Links Lysosomal Function and Iron Homeostasis to 3' UTR-Directed Regulation and Nonsense-Mediated Decay.
Screen Details:
- Screen Rationale : Regulation of Nonsense-mediated decay (NMD)
- Cell Type : Colorectal Cancer Cell Line [BTO:0001616]
- Cell Line : HCT 116 [BTO:0001109, CELLOSAURUS:CVCL_0291]
- Phenotype : Regulation Of Nonsense-mediated Decay (NMD)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 128 | Full Dataset Size: 19,112
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR/Cas9 screen was carried out to identify new components or regulators of Nonsense-mediated decay (NMD) in human cells. The experiment involved expressing an enhanced green fluorescent protein (EGFP) reporter construct targeted for degradation by NMD.
Score Distribution
< 4.2809
> -1.2608