CRISPR Screen Dataset
Rehfeld F (2023) - 1-PMID36753415
Title: CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication.
Screen Details:
- Screen Rationale : Increased SARS-CoV-2 programmed ribosomal frameshifting (PRF)
- Cell Type : Colorectal Cancer Cell Line [BTO:0001616]
- Cell Line : HCT 116 [BTO:0001109, CELLOSAURUS:CVCL_0291]
- Phenotype : Viral Programmed Ribosomal Frameshifting (PRF)
- Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (0.3 MOI)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 212 | Full Dataset Size: 19,112
- Experimental Setup : Virus Exposure | Duration: 10 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide screen was carried out using dual fluorescent reporters of SARS-CoV-2 frameshifting to identify human host factors that regulate programmed ribosomal frameshifting (PRF) of SARS-CoV-2 .
- The top hits are host genes whose loss of function increased the efficiency of frameshifting, thereby representing negative regulators of SARS-CoV-2 PRF.
Score Distribution
< 3.0539
> -1.7441