CRISPR Screen Dataset

Chan K (2022) - 13-PMID36597481

H. sapiens

Title: Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors.

Screen Rationale : Increased resistance to virus
Cell Type : African Green Monkey Kidney Cell Line
Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
Phenotype : Response To Virus
Condition : Virus: SARS-CoV-2/SB3-TYAGNC (0.001 MOI)
Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : Quantitative genetic interaction (qGI) method (Chan, 2022) | Number of Hits: 30 | Full Dataset Size: 11,783
Experimental Setup : Virus Exposure | Duration: 21 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.1 (Differential score) > 0.0 AND Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-wide CRISPR screen was carried out to select for host factors required for SARS-CoV-2 cytotoxicity. Positive hits were identified using a modified quantitative genetic interaction (qGI) scoring method. None of the identified gene hits selected by SARS-CoV-2 exposure were identified as essential fitness genes in the accompanying cell-essential CRISPR screen in the same cell line. (Virus exposure for 21 days at MOI = 0.001.)

< 9.325338733

> -3.134307125