CRISPR Screen Dataset

Chan K (2022) - 3-PMID36597481

H. sapiens

Title: Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors.

Screen Rationale : Cell-essential genes
Cell Type : Hepatoma Cell Line [BTO:0000578]
Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
Phenotype : Cell Proliferation
Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : BAGEL | Number of Hits: 2,093 | Full Dataset Size: 18,052
Experimental Setup : Timecourse | Duration: 15 Doublings | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 5.0 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-wide CRISPR knockout screen was performed to identify context-essential genes within a cell line and core essential genes across five cell lines.

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