CRISPR Screen Dataset
Chan K (2022) - 2-PMID36597481
Title: Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293-ACE2-TMPRSS2
- Phenotype : Cell Proliferation
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 2,058 | Full Dataset Size: 18,051
- Experimental Setup : Timecourse | Duration: 15 Doublings | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 5.0 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR knockout screen was performed to identify context-essential genes within a cell line and core essential genes across five cell lines.
- This screen was carried out using HEK293 cells transduced with human ACE2 and TMPRSS2 (HEK293 +A+T cells).
Score Distribution
< 62.216
> -262.114