CRISPR Screen Dataset
Song J (2022) - 2-PMID36228039
Title: LRRC15 inhibits SARS-CoV-2 cellular entry in trans.
Screen Details:
- Screen Rationale : Identifying cell surface proteins binding the SARS-CoV-2 spike protein
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
- Phenotype : Protein Binding
- Condition : SARS-CoV-2 Spike-RBD (50.0 µg/mL)
- Library : Surfaceome CRISPRa library (Song, 2022) | Type: CRISPRa | Format: Pool | Enzyme: dCas9 | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 2 | Full Dataset Size: 6,891
- Experimental Setup : SARS-CoV-2 Spike-RBD exposure | Duration: 30 Minutes | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 1.0 AND Score.2 (Log10 (p-value)) > 4.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A focused Surfaceome CRISPRa activation screen was carried out to identify cell surface proteins binding the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. The CRISPRa screen identified human LRRC15 as a novel inhibitory attachment factor for SARS-CoV-2 entry. (Replicate 2 screen)
Score Distribution
< 1.741753497
> -0.8670213744